| Data | In casa | Ospite | Risultato |
|---|---|---|---|
| 20/12/22 19.00 | CF Intercity | CD Mirandes | 2:0 |
| 20/12/22 19.00 | CD Diocesano | FC Getafe | 0:2 |
| 20/12/22 19.00 | CD Guadalajara | FC Elche | 0:3 |
| 20/12/22 21.00 | AD Alcorcon | FC Cartagena | 3:5d.c.r |
| 20/12/22 21.00 | CD Guijuelo | FC Villarreal | 1:2d.t.s |
| 20/12/22 21.00 | Sestao River Club | Athletic Bilbao | 0:1 |
| 20/12/22 21.00 | Real Union Club | RCD Mallorca | 0:1 |
| 20/12/22 22.00 | CD Atletico Paso | Espanyol Barcellona | 0:1 |
| 21/12/22 19.00 | UD Levante | Andorra CF | 2:1 |
| 21/12/22 19.00 | CD Numancia | Sporting Gijon | 0:3 |
| 21/12/22 19.00 | Merida AD | Deportivo Alaves | 0:1 |
| 21/12/22 19.00 | CD Ibiza Islas Pitiusas | SD Eibar | 1:0 |
| 21/12/22 19.00 | Atletico Saguntino | Rayo Vallecano | 1:3d.c.r |
| 21/12/22 21.00 | CF Pontevedra | CD Teneriffa | 2:1 |
| 21/12/22 21.00 | AD Ceuta FC | UD Ibiza Eivissa | 3:2 |
| 21/12/22 21.00 | UD Logrones | Albacete Balompie | 4:3d.c.r |
| 21/12/22 21.00 | CD Coria | Real Sociedad | 0:5 |
| 21/12/22 21.00 | Juventud Torremolinos CF | FC Siviglia | 0:3 |
| 21/12/22 21.00 | Arnedo | CA Osasuna | 1:3 |
| 21/12/22 21.00 | Arenas Club de Getxo | Real Valladolid | 1:5 |
| 22/12/22 19.00 | Eldense | Burgos CF | 1:0 |
| 22/12/22 19.00 | Linares Deportivo | Racing Santander | 1:0 |
| 22/12/22 19.00 | CP Cacereno | FC Girona | 2:1 |
| 22/12/22 19.00 | SD Gernika Club | Celta Vigo | 0:3 |
| 22/12/22 21.00 | Real Oviedo | FC Granada | 1:0 |
| 22/12/22 21.00 | CF La Nucia | UD Las Palmas | 6:5d.c.r |
| 22/12/22 21.00 | Gimnastica Segoviana | FC Malaga | 2:1 |
| 22/12/22 21.00 | CD Arenteiro | Atletico Madrid | 1:3 |